P-Selectin (Soluble) (CD62) Human ELISA Kit ELISA试剂盒,P-Selectin (Soluble) (CD62) Human ELISA Kit/人P选择素（可溶）（CD62）ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

P-Selectin (Soluble) (CD62) Human ELISA Kit ELISA试剂盒,P-Selectin (Soluble) (CD62) Human ELISA Kit/人P选择素（可溶）（CD62）ELISA试剂盒

2024-10-23

P-Selectin (Soluble) (CD62) Human ELISA Kit/人P选择素（可溶）（CD62）ELISA试剂盒

货号：BMS219-4,BMS219-4TEN

规格：96 Tests,10X96 Tests

价格：1,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.20 ng/mL
检测范围	0.63-40.00 ng/mL
样本类型/体积	血清 10 μL 血浆 10 μL 羊水 10 μL 上清液 10 μL
操作时间	1 hr 20 min
测试时间	2 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	5.4%
批内变异系数（CV）	7.8%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - HRP偶联检测抗体 - 洗涤液 - 对照 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	P-Selectin (CD62P)
Protein aliases	CD62 antigen-like family member P, CD62P, GMP-140, Granule membrane protein 140, granule membrane protein 140kDa, granulocyte membrane protein, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, P-selectin, PADGEM, Platelet activation dependent granule-external membrane protein, platelet alpha-granule membrane protein, selectin P (granule membrane protein 140kDa, antigen CD62)
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	CD62, CD62P, GMP140, GMRP, GRMP, LECAM3, PADGEM, PSEL, SELP
Gene ID	(Human) 6403
Gene symbol	SELP
UniProt ID	(Human) P16109

The Human Soluble P-Selectin (Hu sP-Sel/CD62)) ELISA quantitates Hu sP-Selectin in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sP-Selectin.人可溶性P-选择素（人sP-Sel / CD62）ELISA定量分析人血清，血浆，羊水或细胞培养基中的人sP-选择素。该检测试剂盒将只识别天然和重组人sP-选择素。Principle of the method 方法原理The Human sP-Selectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人P选择素固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.P-选择素（Selectin P，GMP-140，LECAM3，CD62抗原样家族成员）是一种140 kDa的1型跨膜糖蛋白，是调节细胞滚动的最常研究的蛋白之一。 P-选择素储存在内皮细胞的Weibel-Palade体以及血小板的a颗粒中。暴露于凝血酶，组胺，补体5a，Ca21离子载体或二磷酸腺苷后，可以从那里迅速将其带到细胞表面。 P-选择素蛋白在血小板活化和脱粒过程中重新分布到质膜，并介导活化的内皮细胞或血小板与白细胞的相互作用。 P-选择蛋白在白细胞与内皮细胞之间的黏附过程中起重要作用，并且是钙依赖性受体，可与中性粒细胞和单核细胞上发现的路易斯血型碳水化合物抗原的唾液酸化形式结合。 P-选择蛋白在炎症中组成性表达，并有助于动脉粥样硬化，血栓形成和组织破坏。在临床上，P-选择素用于区分有或没有血栓形成的肝素诱导的血小板减少症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

P-Selectin (Soluble) (CD62) Human ELISA KitRepresentative standard curve for Human P-Selectin ELISA

技术参数

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P-Selectin (Soluble) (CD62) Human ELISA Kit ELISA试剂盒,P-Selectin (Soluble) (CD62) Human ELISA Kit/人P选择素（可溶）（CD62）ELISA试剂盒

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