PSGL-1/CD162 (Soluble) Human ELISA Kit ELISA试剂盒,PSGL-1/CD162 (Soluble) Human ELISA Kit/人PSGL-1 / CD162(可溶)ELISA试剂盒

2024-10-23

PSGL-1/CD162 (Soluble) Human ELISA Kit/人PSGL-1 / CD162(可溶)ELISA试剂盒

货号:BMS255,BMS255TEN

规格:96 Tests,10X96 Tests

价格:1,34832

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

1.0 U/mL

检测范围1.56-50 U/mL

样本类型/体积

血清 15 μL

上清液 100 μL

操作时间

1 hr 20 min

测试时间

3 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)6.6%
批内变异系数(CV)3.2%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 对照

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameCD162 (PSGL-1)
Protein aliasesCD162, cutaneous lymphocyte-associated associated antigen, P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCD162, CLA, PSGL-1, PSGL1, SELPLG
Gene ID(Human) 6404
Gene symbolSELPLG
UniProt ID(Human) Q14242

The Human P-selectin Glycoprotein Ligand-1 (Hu PSGL-1/CD162) ELISA quantitates Hu PSGL-1/CD162 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PSGL-1/CD162.人P选择素糖蛋白配体1(人PSGL-1 / CD162)ELISA定量了人血清或细胞培养基中的人PSGL-1 / CD162。 该检测试剂盒将专门识别天然和重组人PSGL-1 / CD162。Principle of the method 方法原理The Human PSGL-1/CD162 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人PSGL-1 / CD162固相夹心ELISA(酶联免疫吸附测定)旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets. CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin). These interactions play a critical role in leukocyte adhesion, rolling and extravasation. CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.CD162(PSGL-1)是在白细胞和血小板表面发现的120 kDa同型二聚体蛋白。 已知CD162可以结合CD62P(P-选择素),CD62L(L-选择素)和较低程度的CD62E(E-选择素)。 这些相互作用在白细胞粘附,滚动和外渗中起关键作用。 在慢性病毒感染和癌症中,CD162已被证明是T细胞衰竭的重要免疫标记。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

PSGL-1/CD162 (Soluble) Human ELISA KitRepresentative standard curve for Human PSGL-1 ELISA

技术参数
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