Cathepsin L Human ELISA Kit ELISA试剂盒,Cathepsin L Human ELISA Kit/人组织蛋白酶L ELISA试剂盒

2024-10-23

Cathepsin L Human ELISA Kit/人组织蛋白酶L ELISA试剂盒

货号:BMS257,BMS257TEN

规格:96 Tests,10X96 Tests

价格:1,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

1.7 ng/mL

检测范围3.1-50 ng/mL

样本类型/体积

血清 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

3 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)6%
批内变异系数(CV)6.2%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 裂解液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameCathepsin L
Protein aliasesCathepsin L, Major excreted protein, MEP, Cathepsin L1, Cathepsin L1 heavy chain, Cathepsin L1 light chain

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCATL, CTSL, CTSL1, MEP
Gene ID(Human) 1514
Gene symbolCTSL
UniProt ID(Human) P07711

The Human Cathepsin L ELISA quantitates Hu Cathepsin L in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Cathepsin L.人组织蛋白酶L ELISA可以定量人血清或细胞培养基中的人组织蛋白酶L。 该检测试剂盒将专门识别天然和重组的人组织蛋白酶L。Principle of the method 方法原理The Human Cathepsin L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人组织蛋白酶L固相夹心ELISA(酶联免疫吸附测定)旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.该基因编码的蛋白质是溶酶体半胱氨酸蛋白酶,在细胞内蛋白质分解代谢中起主要作用。 它的底物包括胶原蛋白和弹性蛋白,以及中性粒细胞弹性蛋白酶活性的主要控制元素α-1蛋白酶抑制剂。 所编码的蛋白质与多种病理过程有关,包括肌病和心肌缺血中的肌原纤维坏死,以及对蛋白尿的肾小管反应。 该蛋白是肽酶C1家族的成员,是由二硫键连接的重链和轻链组成的二聚体,均由单一蛋白前体产生。 已经发现该基因至少有两个编码相同蛋白质的转录物变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

Cathepsin L Human ELISA KitRepresentative standard curve for Human Cathepsin ELISA

技术参数
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