s90K/Mac-2BP (Soluble) Human ELISA Kit ELISA试剂盒,s90K/Mac-2BP (Soluble) Human ELISA Kit/人s90K / Mac-2BP(可溶)ELISA试剂盒

2024-10-23

s90K/Mac-2BP (Soluble) Human ELISA Kit/人s90K / Mac-2BP(可溶)ELISA试剂盒

货号:BMS234

规格:96 Tests

价格:1

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.92 ng/mL

检测范围12.5-200 ng/mL

样本类型/体积

血清 10 μL

血浆 10 μL

上清液 10 μL

操作时间

1 hr 20 min

测试时间

1 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)11.9%
批内变异系数(CV)5.5%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- HRP偶联检测抗体

- 洗涤液

- 对照

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameLGALS3BP
Protein aliasesBasement membrane autoantigen p105, Galectin-3-binding protein, L3 antigen, lectin galactoside-binding soluble 3 binding protein, Lectin galactoside-binding soluble 3-binding protein, lectin, galactoside binding soluble 3 binding protein, galactoside-binding, soluble, 3 binding protein, mac-2 BP, Mac-2-binding protein, MAC2BP, serum protein 90K, transport and golgi organization 10 homolog B, Tumor-associated antigen 90K

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliases90K, BTBD17B, CyCAP, gp90, LGALS3BP, M2BP, MAC-2-BP, TANGO10B
Gene ID(Human) 3959
Gene symbolLGALS3BP
UniProt ID(Human) Q08380

The Human Soluble 90K/Mac-2BP (Hu s90K/Mac-2BP) ELISA quantitates Hu s90K/Mac-2BP in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu s90K/Mac-2BP.人可溶性90K / Mac-2BP(人s90K / Mac-2BP)ELISA定量了人类血清,血浆,缓冲液或细胞培养基中的人s90K / Mac-2BP。 该检测试剂盒将专门识别天然和重组人s90K / Mac-2BP。Principle of the method 方法原理The Human s90K/Mac-2BP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人s90K / Mac-2BP固相夹心ELISA(酶联免疫吸附测定)旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1.半乳凝素是一类β-半乳糖苷结合蛋白,与调节细胞-细胞和细胞-基质相互作用有关。 已经发现,在患有癌症的患者和被人类免疫缺陷病毒(HIV)感染的患者的血清中,LGALS3BP升高。 它似乎与自然杀手(NK)和淋巴因子激活的杀手(LAK)细胞细胞毒性有关的免疫反应有关。 使用荧光原位杂交,全长90K cDNA已定位于染色体17q25。 天然蛋白与称为Mac-2的人类巨噬细胞相关凝集素特异性结合,并与半乳糖凝集素1结合。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

s90K/Mac-2BP (Soluble) Human ELISA KitRepresentative standard curve for Human 90K/Mac-2 BP ELISA

技术参数
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