VCAM-1 (Soluble) Human ELISA Kit ELISA试剂盒,VCAM-1 (Soluble) Human ELISA Kit/人VCAM-1(可溶性)ELISA试剂盒

2024-10-23

VCAM-1 (Soluble) Human ELISA Kit/人VCAM-1(可溶性)ELISA试剂盒

货号:BMS232,BMS232TEN

规格:96 Tests,10X96 Tests

价格:1,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.6 ng/mL

检测范围3.15-100 ng/mL

样本类型/体积

血清 10 μL

血浆 10 μL

羊水 10 μL

上清液 100 μL

操作时间

1 hr 20 min

测试时间

2 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)5.2%
批内变异系数(CV)3.1%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 对照

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameVCAM-1 (CD106)
Protein aliasesCD106, CD106 antigen, INCAM-100, V-CAM 1, VCAM-1, Vascular cell adhesion protein 1

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCD106, INCAM-100, VCAM1
Gene ID(Human) 7412
Gene symbolVCAM1
UniProt ID(Human) P19320

The Human Vascular Cell Adhesion Molecula-1 (Hu VCAM-1) ELISA quantitates Hu VCAM-1 in human serum, plasma, amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu VcAM-1.人血管细胞粘附分子1(人VCAM-1)ELISA定量分析人血清,血浆,羊水或细胞培养基中的人VCAM-1。 该检测试剂盒将专门识别天然和重组人VcAM-1。Principle of the method 方法原理The Human VCAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人VCAM-1固相夹心ELISA(酶联免疫吸附测定)旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.VCAM-1是由细胞因子激活的内皮表达的细胞表面唾液酸糖蛋白。 该蛋白具有许多功能,包括调节白细胞迁移,白细胞-内皮细胞粘附和信号转导,并且可能在许多炎性疾病中起作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

VCAM-1 (Soluble) Human ELISA KitRepresentative standard curve for Human VCAM-1 ELISA

技术参数
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