CRP Rat ELISA Kit ELISA试剂盒,CRP Rat ELISA Kit/大鼠CRP ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

CRP Rat ELISA Kit ELISA试剂盒,CRP Rat ELISA Kit/大鼠CRP ELISA试剂盒

2024-10-23

CRP Rat ELISA Kit/大鼠CRP ELISA试剂盒

货号：88-7501-28

规格：96 tests

价格：1

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：大鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	2 ng/mL
检测范围	2-133 ng/mL
样本类型/体积	血清 1 μL 血浆 1 μL 尿液 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 tests
组份	- 预包被96孔板 - 标准品 - 检测抗体 - 洗涤缓冲粉 - 底物溶液 - 终止液
储存温度	2-8℃
Protein name	CRP
Protein aliases	C-reactive protein, C-reactive protein member of the pentraxin family, member of the pentraxin family, petaxin related
物种（Tested）	大鼠
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	Aa1249, Ab1-341, Ab2-196, Ac1-114, Ac1262, Ac2-069, Ba2-693, Crp, Ptx1
Gene ID	(Rat) 25419
Gene symbol	Crp
UniProt ID	(Rat) P48199

The Rat C-Reactive Protein (CRP) ELISA quantitates Rt CRP in rat serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt CRP.大鼠C反应蛋白（CRP）ELISA对大鼠血清，血浆，尿液或细胞培养基中的大鼠CRP进行定量。该检测试剂盒将专门识别天然和重组大鼠CRP。Principle of the method 方法原理The Rt CRP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠CRP固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息CRP (C-reactive protein) is a major cyclic, pentameric acute phase protein compound consisting of five identical, noncovalently bound, nonglycosylated subunits. Eeach subunit is made up of 206 amino acids, and has a molecular weight of 24 kDa. CRP is produced by the liver, and its plasma levels rise dramatically during inflammatory processes occurring in the body. CRP is an initiator of classical complement cascade, binds to several nuclear components (chromatin, histones, etc.), plays an important role in innate immunity, and mediates activities associated with pre-immune nonspecific host resistance. Specifically, there is a 100-1000 fold increase of CRP in response to injury, infection or inflammation. CRP is opsonic, an initiator of the classical complement cascade, and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. CRP is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host, and initiates pathogen elimination by interacting with humoral and cellular effector systems in the blood. Patients with elevated basal levels of CRP have been shown to be an increased risk for hypertension and cardiovascular disease.CRP（C反应蛋白）是一种主要的环状五聚体急性期蛋白化合物，由五个相同的，非共价结合的，非糖基化的亚基组成。每个亚基由206个氨基酸组成，分子量为24 kDa。 CRP由肝脏产生，在体内发生炎症过程中血浆CRP急剧升高。 CRP是经典补体级联反应的引发剂，与多种核成分（染色质，组蛋白等）结合，在先天免疫中起重要作用，并介导与免疫前非特异性宿主抗性相关的活性。具体而言，响应于损伤，感染或炎症，CRP增加了100-1000倍。 CRP是调渗的，是经典补体级联反应的引发剂，也是单核细胞/巨噬细胞的活化剂。 CRP还与包括染色质，组蛋白和snRNP在内的几个核成分结合，表明它可能在细胞坏死过程中起清除剂的作用。 CRP基于其识别宿主的外来病原体和受损细胞的能力，并通过与血液中的体液和细胞效应系统相互作用而启动病原体消除，从而参与了几种与宿主防御相关的功能。已显示基础CRP水平升高的患者患高血压和心血管疾病的风险增加。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

CRP Rat ELISA KitRepresentative standard curve for rat CRP ELISA

技术参数

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CRP Rat ELISA Kit ELISA试剂盒,CRP Rat ELISA Kit/大鼠CRP ELISA试剂盒

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