LYVE-1 Human Uncoated ELISA Kit with plates/人LYVE-1未包被ELISA试剂盒
货号:88-52239-22
规格:2 x 96 Tests
价格:1
产品类型:自定义ELISA
品牌:Thermo Fisher
物种:人
宿主:其它
抗体亚型:其它
荧光染料:其它
灵敏度 | 1.09 pg/mL |
检测范围 | 1.09-70 pg/mL |
样本类型/体积 | 血清 50 μL 血浆 50 μL 上清液 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 3 hr 15 min |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 2 x 96 Tests |
组份 | - 捕获抗体 - 检测抗体 - 包被缓冲液 - 标准品(2小瓶) - 标准稀释液 - 生物素化检测抗体 - 链霉亲和素-HRP - 洗涤液 - 显色剂 - 96孔板(2) |
储存温度 | 2-8℃ |
Protein name | LYVE1 |
Protein aliases | cell surface retention sequence binding protein-1, Cell surface retention sequence-binding protein 1, CRSBP-1, extracellular link domain containing 1, extracellular link domain-containing 1, Extracellular link domain-containing protein 1, Hyaluronic acid receptor, Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE-1 |
物种(Tested) | 人 |
试剂盒类型 | Uncoated ELISA Kit with Plates |
标记(染料) | HRP |
检测抗体偶联物 | Biotin |
Gene aliases | CRSBP-1, CRSBP1, HAR, LYVE-1, LYVE1, UNQ230/PRO263, XLKD1 |
Gene ID | (Human) 10894 |
Gene symbol | LYVE1 |
UniProt ID | (Human) Q9Y5Y7 |
The Human LYVE-1 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human LYVE-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人LYVE-1未包被ELISA试剂盒包含预先匹配的抗体对和试剂,用于进行定量酶联免疫吸附测定(ELISA),以检测和定量人LYVE-1的蛋白质水平。 需要使用洗涤液和终止液来完成ELISA反应,并且需要单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA被设计为测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被在微孔板孔的底部,这是一整夜的过程。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,该第二抗体与捕获抗体在不同表位上的靶标结合。 已将结合酶掺入测定中。 经过孵育和洗涤步骤以去除未结合物质的微孔板后,添加底物溶液,该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module, the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. LYVE1 is a type I integral membrane glycoprotein. LYVE-1 is expressed primarily on lymphatic vessel endothelium and is likely to be involved in regulating the traffic of leucocytes and tumor cells to lymph nodes. The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. A number of molecules have been identified as markers for lymphatic endothelium which include LYVE1, PALE, VEGFR3, and podoplanin. Diseases associated with LYVE1 dysfunction includes Complete Androgen Insensitivity Syndrome.LYVE1已被确定为淋巴管壁上HA(细胞外基质糖胺聚糖透明质酸)的主要受体。推导的LYVE1氨基酸序列预测与CD44 HA受体相似,具有322个残基的I型整合膜多肽41%,具有212个残基的胞外域,其中包含一个Link模块,即Link蛋白超家族的原型HA结合域。像CD44一样,LYVE1分子结合可溶性HA和固定HA。但是,与CD44不同,LYVE1分子与HA在淋巴管壁腔表面共定位,并且在血管中完全不存在。因此,LYVE1是第一个被表征的淋巴特异性HA受体,并且是淋巴管自身的独特强大标记。 LYVE1是I型整合膜糖蛋白。 LYVE-1主要在淋巴管内皮细胞上表达,可能参与调节白细胞和肿瘤细胞向淋巴结的运输。淋巴管系统形成了第二个循环系统,该循环系统从组织中排出细胞外液,并提供了一个独特的环境,免疫细胞可以在该环境中遇到并响应外来抗原。已鉴定出许多分子作为淋巴管内皮的标记物,包括LYVE1,PALE,VEGFR3和Podoplanin。与LYVE1功能障碍有关的疾病包括完全雄激素不敏感综合症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。
©
bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:
https://gibcohyclone.cn/thread-45753.htm
特别声明:以上内容(如有图片亦包括在内)来源于授权厂家或网络,如有侵权,请联系删除,本平台不提供信息存储服务。
Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.