Bovine IL-21 ELISA Kit ELISA试剂盒,Bovine IL-21 ELISA Kit/牛IL-21 ELISA试剂盒

2024-10-24

Bovine IL-21 ELISA Kit/牛IL-21 ELISA试剂盒

货号:EB11RB,EB11RBX5,EB11RBX10

规格:96 Tests,5X96 Tests,10X96 tests

价格:6641,29884,57271

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.41 ng/mL

检测范围0.41-100 ng/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 Tests

组份

- 预包被96孔板

- 标准品

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameIL-21
Protein aliasesIL-21, Interleukin-21

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesIL21
Gene ID(Bovine) 378475
Gene symbolIL21
Bovine IL-21 quantitates bovine IL-21 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant bovine IL-21.牛IL-21定量测定血清,血浆,上清液中的牛IL-21。该检测试剂盒将只识别天然和重组牛IL-21。Target information 靶标信息IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.IL-21是一种17 kDa的免疫调节细胞因子,主要由自然杀伤细胞(NKT),T辅助(Th)17和T滤泡辅助(TFH)细胞产生。在TFH细胞中,IL-21表达导致通过IL-21受体(IL-21R)和STAT3的自分泌信号传导,从而导致Bcl6进行额外的转录激活。与Th1细胞的IFNγ和Th2细胞的IL-4一样,IL-21对于TFH细胞发育和效应子功能至关重要。 IL-21在依赖T细胞的B细胞分化为浆细胞和记忆细胞,刺激IgG产生以及诱导天然B细胞中凋亡信号转导中发挥作用。在Th17细胞中,IL-21的表达和通过STAT3,IRF4和RORγt引起的自分泌反馈导致IL-23R上调,从而为Th17细胞做好准备,使其通过炎性细胞因子IL-23进行成熟和维持。 IL-21在上调IRF4和RORγt的同时,还介导Foxp3的下调。 IL-21缺陷小鼠由于无法上调与Th17相关的分子而受到化学治疗,从而免受结肠炎的侵袭。相比之下,化学诱导的结肠炎小鼠模型中存在IL-21的升高水平。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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