c-Jun Recombinant Polyclonal Antibody (2HCLC)/c-Jun重组多克隆抗体（2HCLC）

货号：711202

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to Human JUN (aa 21-36, 211

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：2HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg x 10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Bovine, Pig and Mouse.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

数据：

c-Jun Antibody (711202) in IFImmunofluorescence analysis was performed on fixed and permeabilized PC-3 cells for detection of endogenous c-jun using Anti-c-jun Recombinant Rabbit Polyclonal Antibody (Product # 711202, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of c-jun protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of c-jun. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. c-Jun Antibody (711202) in ChIPEnrichment of endogenous c-Jun protein at specific gene loci using Anti-c-Jun Recombinant Rabbit Polyclonal Antibody: Chromatin immunoprecipitation (ChIP) was performed using Anti-c-Jun Recombinant Rabbit Polyclonal Antibody (Product # 711202, 5 µg) on sheared chromatin from 2 million U-937 cells and vs U-937 cells treated with 250 ng/mL anisomycin for 30 minutes using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active JAG1, MMP13 region used as positive control target genes, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is compared between untreated vs treated samples and is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method. c-Jun Antibody (711202) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of COLO 205 (Lane 1), U-937 (Lane 2), NIH/3T3 (Lane 3), HEK-293 (Lane 4) and PC-3 (Lane 5). The blots were probed with Anti-c-jun Recombinant Rabbit Polyclonal Antibody (Product # 711202, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 43 kDa band corresponding to c-jun was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

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参考文献：

1. Molecular medicine reportsc‑Jun/Ap‑1 is upregulated in an Ang II‑induced abdominal aortic aneurysm formation model and mediates Chop expression in mouse aortic smooth muscle cells.

技术参数

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