APE1 Monoclonal Antibody (13B 8E5C2)/APE1单克隆抗体（13B 8E5C2）

货号：MA1-440

规格：100 µL

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Full-length recombinant human APE protein.

物种：其它

宿主：小鼠

抗体亚型：其它

克隆号：13B 8E5C2

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1.5 µg/10^6 cells(ChIP)；2-5 µg/mL(Flow)；1:50-1:500(ICC)；1:50-1:500(IF)；1/5000(IHC (F))；1:50-1:1000(IHC (P))；5 µg(IP)；1:1,000(WB)

产品详细信息MA1-440 detects apurinic/apyrimidinic endonuclease (APE/ref-1) from human, non human primate, canine, mouse and rat samples.MA1-440 has been successfully used in Western blot, immunohistochemical, flow cytometry and immunoprecipitation procedures. By Western blot, this antibody detects a 36 kDa protein representing APE/ref-1 in HeLa cell lysate. Immunohistochemical staining of APE/ref-1 in a variety of normal and cancerous human tissues, including ovaries, cervix, prostate, germ cell tumors, osteosarcomas, and rhabdosarcomas, with MA1-440 yields a staining pattern consistent with nuclear staining.The MA1-440 immunogen is full-length, recombinant human APE protein.靶标信息Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which arebrought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.

数据：

APE1 Antibody (MA1-440) in IFImmunofluorescent analysis of APE1 (green) showing staining in the in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an APE1 monoclonal antibody (Product # MA1-440) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. APE1 Antibody (MA1-440) in IHC (P)Immunohistochemistry analysis of APE1 showing staining in the nucleus of paraffin-embedded human prostate carcinoma (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with an APE1 monoclonal antibody (Product # MA1-440) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. APE1 Antibody (MA1-440) in FlowFlow cytometry analysis of APE1 was done on HeLa cells. The cells were fixed, permeabilized and stained with a APE1 mouse monoclonal antibody (Product # MA1-440, red histogram) or Mouse IgG2a isotype control (Product # MA1-10418, black histogram) at a concentration of 2.5 µg/mL. After incubation of the primary antibody on ice for an hour, the cells were stained with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 680 conjugate (Product # A32734) at a dilution of 1:50 for at least 30 minutes on ice. A representative 10,000 cells were acquired for each sample.

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参考文献：

1. Clinical cancer research : an official journal of the American Association for Cancer ResearchStudies of apurinic/apyrimidinic endonuclease/ref-1 expression in epithelial ovarian cancer: correlations with tumor progression and platinum resistance.

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