FLI1 Monoclonal Antibody (7.3) /FLI1单克隆抗体（7.3）

货号：MA1-196

规格：100 µL

价格：4810

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Recombinant human DHFR-FLICTER (C-terminal region

物种：人/小鼠

宿主：小鼠

抗体亚型：其它

克隆号：7.3

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	Assay-Dependent(ChIP)；1:100(ICC)；1:100(IF)；1:100(IHC)；1:500(WB)

产品详细信息MA1-196 detects FLI-1 in human and mouse samples.MA1-196 has been successfully used in western blot, immunofluorescence and ChIP assays.靶标信息ETS-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes ETS-1, ETS-2, ERG-1, ERG-2, ELK, E74, FLI-1, PU. 1 and PEA3. Members of the ETS gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

数据：

FLI1 Antibody (MA1-196) in IFImmunofluorescent analysis of FLI-1 (green) in Ramos cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without monoclonal antibody (Product # MA1-196), at a dilution of 1:100 overnight at 4°C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification. FLI1 Antibody (MA1-196) in ChIPChromatin immunoprecipitation analysis of HIV1 FLI-1 was performed using cross-linked chromatin from Human 5A8 J-lat T lymphocytes culture latently infected with HIV1 and treated with 10 µg/mL PHA (phytohemaglutin) for 0, 2, and 20 hours. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay with 1.0 µL/100 µL well volume of a FLI-1 monoclonal antibody (Product # MA1-196). Chromatin aliquots from 1 x 105 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers shown to amplify exon-1 of the EGR1 gene or HIV ENV gene. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. Schematic representations of the EGR-1 and HIV1 ENV locus are shown above the data where boxes represent exons (black boxes equal translated regions, white boxes equal untranslated regions, the zigzag line represents an intron) and the straight line represents upstream sequence. Regions amplified by the primers are represented by black bars. Data courtesy of the Innovators Program.

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