FOXA2 Recombinant Rabbit Monoclonal Antibody (9H5L7)/FOXA2重组兔单克隆抗体（9H5L7）

货号：701698

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Protein corresponding to Human FOXA2 (aa 236-451)

物种：人

宿主：兔

抗体亚型：IgG

克隆号：9H5L7

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	2-3 µg/1x10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Horse, Cat and Dog.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin. They also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA2 has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified.

数据：

FOXA2 Antibody (701698) in IFImmunofluorescence was performed on methanol fixed HepG2 cells for detection of FOXA2 using FOXA2 Recombinant Rabbit Monoclonal Antibody (Product # 701698, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Cytoskeleton was stained with alpha-Tubulin Monoclonal Antibody (Product # 32-2500, 1 µg/mL) followed by Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A-11032, 1:400). Panel a) shows representative cells that were stained for detection and localization of FOXA2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal alpha-Tubulin staining (red). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of FOXA2. Panel e) represents control cells with no primary antibody to assess background. FOXA2 Antibody (701698) in ChIPEnrichment of endogenous FOXA2 protein using Anti-FOXA2 Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-FOXA2 Recombinant Rabbit Monoclonal Antibody (Product # 701698, 5 µg) on sheared chromatin from 2 million HepG2 cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active HNF1A region used as positive control target gene, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method. FOXA2 Antibody (701698) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), PC-3 (Lane 2) and COLO 205 (Lane 3). The blots were probed with Anti-FOXA2 Recombinant Rabbit Monoclonal Antibody (Product # 701698, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 48 kDa band corresponding to FOXA2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

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