Gata-3 Monoclonal Antibody (TWAJ), Alexa Fluor 488, eBioscience™/Gata-3 单抗 (TWAJ), Alexa Fluor 488, eBioscience™

货号：53-9966-42,53-9966-41

规格：100 tests,25 tests

价格：3797,1526

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：TWAJ

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor 488, eBioscience™
浓度：	5 µL/Test	用法：	1:250(ICC);1:250(IF);5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation.Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(cat 00-5523)Crossreactivity in rhesus monkeys has been published.Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This TWAJ antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis inT cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Gata-3 Antibody (53-9966-42) in Flow BALB/c thymocytes were surface stained with Anti-Mouse CD4 PE-Cyanine7 (Product # 25-0042-82) and Anti-Mouse CD8a eFluor® 450 (Product # 48-0081-82) followed by intracellular staining with Rat IgG2b K Isotype Control Alexa Fluor® 488 (Product # 53-4031-80) (blue histogram) or Anti-Human/Mouse Gata-3 Alexa Fluor® 488 (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the CD4 single positive (left) or CD8 single positive (right) gates were used for analysis.

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参考文献：

1.Wellcome open researchCharacterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity.2.NatureStability and function of regulatory T cells expressing the transcription factor T-bet.3.PloS oneIntranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced Inflammation by Targeting GATA-3.4.The Journal of investigative dermatologyImpaired proteasome function activates GATA3 in T cells and upregulates CTLA-4: relevance for Sézary syndrome.5.Journal of immunology (Baltimore, Md. : 1950)Role of CD44 in the differentiation of Th1 and Th2 cells: CD44-deficiency enhances the development of Th2 effectors in response to sheep RBC and chicken ovalbumin.

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