HMGA2 Polyclonal Antibody/HMGA2多克隆抗体

货号：PA5-21320

规格：100 µL

价格：5819

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Recombinant fragment corresponding to a region wit

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.083 mg/mL	用法：	Assay-dependent(ChIP)；1:100-1:1000(ICC)；1:100-1:1000(IF)；1:100-1:1000(IHC (P))；1:100-1:500（IP）；2-3 µg/mL(WB)

产品详细信息Recommended positive controls: A431, HeLa, HepG2, HCT116.Predicted reactivity: Mouse (95%), Rat (92%), Zebrafish (85%), Xenopus laevis (80%), Chicken (98%).Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 shares 95% amino acid sequence identity.

数据：

HMGA2 Antibody (PA5-21320) in IFImmunofluorescent analysis of HMGA2 in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a HMGA2 polyclonal antibody (Product # PA5-21320). Green: Primary antibody at a dilution of 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm. HMGA2 Antibody (PA5-21320) in IHC (P)Immunohistochemical analysis of HMGA2 in paraffin-embedded rat brain tissue using a polyclonal HMGA2 antibody (Product # PA5-21320) at a dilution of 1:500. HMGA2 Antibody (PA5-21320)Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. HeLa cells were transfected with HMGA2 shRNA and decrease in signal intensity was observed in western blot application using HMGA2 antibody (Product # PA5-21320).

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