JunB Recombinant Rabbit Monoclonal Antibody (15H19L4)/JunB重组兔单克隆抗体（15H19L4）

货号：701702

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to Human JUNB (aa 6-25, 262

物种：人

宿主：兔

抗体亚型：IgG

克隆号：15H19L4

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	2-4 µg x 10^6 cells(ChIP)；1-2 µg/mL(ICC)；1-2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Rat, Mouse and Feline.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency,animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息JunB, a member of the Jun cellular oncogene family, is a basic region, leucine zipper transcription factor. It is involved in regulating gene activity following the primary growth factor response. JunB binds to the DNA sequence 5'-TGA[CG]TCA-3'. Cellular oncogenes play critical roles in cellular communication pathways that regulate growth, development, and differentiation.

数据：

JunB Antibody (701702) in IFImmunofluorescence was performed on fixed and permeabilized HeLa cells for detection of JUNB using Anti-JUNB Recombinant Rabbit Monoclonal Antibody (Product # 701702, 1-2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of JUNB protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of JUNB. Panel e) represents control cells with no primary Antibody to assess background. JunB Antibody (701702) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of Hela (Lane1), U-87MG (Lane 2), A431 (Lane 3), PC3 (Lane 4) and K562 (Lane 5). The blots were probed with Anti-JUNB Recombinant Rabbit Monoclonal Antibody (Product # 701702, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 36 kDa band corresponding to JUNB was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). JunB Antibody (701702) in ChIPChromatin Immunoprecipiation (ChIP) was performed using Anti-JunB Recombinant Rabbit Monoclonal Antibody (Product # 701702, 5 µg) on sheared chromatin from 2 million HeLa cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active MMP13, CREB5 region used as positive control target gene, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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