JunB Recombinant Polyclonal Antibody/JunB重组多克隆抗体

货号：710734

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to Human JUNB (aa 6-25, 262

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg/million cells(ChIP)；1 µg/mL(ICC)；1 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Rat, Mouse, Cat and Bovine.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息JunB, a member of the Jun cellular oncogene family, is a basic region, leucine zipper transcription factor. It is involved in regulating gene activity following the primary growth factor response. JunB binds to the DNA sequence 5'-TGA[CG]TCA-3'. Cellular oncogenes play critical roles in cellular communication pathways that regulate growth, development, and differentiation.

数据：

JunB Antibody (710734) in IFImmunofluorescence was performed on fixed and permeabilized HeLa cells for detection of JunB using Anti-Jun B Recombinant Rabbit Polyclonal Antibody (Product # 710734, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of JunB protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of JunB Panel e) represents control cells with no primary Antibody to assess background. JunB Antibody (710734) in WBKnockdown of Jun B was achieved by transfecting HeLa cells with Jun B specific siRNAs (Silencer® select Product # s223956 and s7663). Western blot analysis (Fig a) was performed using whole cell lysates from the Jun B knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Jun B Recombinant Rabbit Polyclonal Antibody (Product # 710734, 1-2 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to Jun B.

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