JunD Recombinant Polyclonal Antibody (2HCLC)/JunD重组多克隆抗体（2HCLC）

货号：710701

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptide, multiple (2-16, 132-149, 299-313)

物种：人

宿主：兔

抗体亚型：IgG

克隆号：2HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	3-5 µg/mL(ChIP)；3-5 µg/mL(ICC)；3-5 µg/mL(IF)；2-3 µg/mL(WB)

产品详细信息This antibody is predicted to react with monkey, sheep, mouse, rat, zebra fish and bovine based on sequence homology.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息JunD is a transcription factor which is coded by JunD gene. JunD transcriptional activity is modulated by phosphorylation in response to cellular stress via the c-Jun N terminal kinase (JNK)/Stress-Activated Protein Kinase (SAPK) family of protein kinase. Jun proteins are capable of forming dimers with Fos, ATF and CREB family transcription factors to form the AP-1 complex that differentially regulates a variety of target genes involved in cellular growth, proliferation, differentiation and apoptosis. Also, JunD plays an important role in metabolism via modulation of IGF-1 signaling pathway.

数据：

JunD Antibody (710701) in IFImmunofluorescent analysis of JunD was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with JunD Recombinant Rabbit Polyclonal Antibody (Product # 710701) at 5 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear localization. Panel e shows no primary antibody control. The images were captured at 20X magnification. JunD Antibody (710701) in WBWestern blot analysis of JunD was performed by loading 50 µg of HeLa (Lane1), A549 (Lane2), Panc1 (Lane 3), HepG2 (Lane 4), K562 (Lane5), Caco2 (Lane6) (Fig: A), K562 (Lane 1), Cos 7 (Lane 2), PC 12 (Lane 3), NIH\u0003T3 (Lane 4), MDCK (Lane 5) (Fig : B) cell lysate using Novex®NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800) and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk 1 hour at room temperature on a rocking platform. JunD was detected at ~42 kDa using JunD Recombinant Rabbit Polyclonal Antibody (Product # 710701) at 2-3 µg/mL in 5 % skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG - HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

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