p53 Monoclonal Antibody (PAb 240)/p53单克隆抗体（PAb 240）

货号：MA5-15244

规格：500 µL

价格：5831

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Murine p53 protein corresponding to aa14-289

物种：其它

宿主：小鼠

抗体亚型：其它

克隆号：PAb 240

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	1-3 µg x 10^6 cells(ChIP)；Assay Dependent(Flow)；2 µg/mL(ICC)；2 µg/mL(IF)；2-4 µg/mL(IHC (P))；2 µg/mL(IP)；1-2 µg/mL(WB)

产品详细信息MA5-15244 targets p53 in FACS, IHC (P), IP, and WB applications and shows reactivity with Human samples.The MA5-15244 immunogen is mouse p53 protein corresponding to aa14-289.靶标信息The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

数据：

p53 Antibody (MA5-15244) in IFImmunofluorescence analysis of p53 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled p53 (PAb 240) Mouse Monoclonal Antibody (Product # MA5-15244) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification. p53 Antibody (MA5-15244) in ChIPEnrichment of endogenous p53 Protein at specific gene loci using Anti-p53 Protein Mouse Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-p53 Protein Mouse Monoclonal Antibody (Product # MA5-15244, 5 µg) on sheared chromatin from 2 million MCF-7 cells, UV treated for 30 minutes using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoter of active CDKN1A, CMBL, HES2, MKN2 gene, used as positive control target, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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参考文献：

1. Nature cell biologyHepatic progenitor cells of biliary origin with liver repopulation capacity.2. Phytomedicine : international journal of phytotherapy and phytopharmacologyThe treatment of skin carcinoma, induced by UV B radiation, using 1-oxo-5beta, 6beta-epoxy-witha-2-enolide, isolated from the roots of Withania somnifera, in a rat model.3. Scientific reportsTP53 transcription factor for the NEDD9/HEF1/Cas-L gene: potential targets in Non-Small Cell Lung Cancer treatment.4. International journal of biological sciencesThe gain of function of p53 mutant p53S in promoting tumorigenesis by cross-talking with H-RasV12.5. Cell proliferationIFI27, a novel epidermal growth factor-stabilized protein, is functionally involved in proliferation and cell cycling of human epidermal keratinocytes.

技术参数

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