p53 Antibody Cocktail/p53抗体混合物

货号：MA5-14067

规格：500 µL

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Recombinant human p53 protein

物种：其它

宿主：小鼠

抗体亚型：其它

克隆号：DO-7, BP53-12

荧光染料：其它

类型:	Cocktail	同型对照：
浓度：	0.125 mg/mL	用法：	1-3 µg x 10^6 cells(ChIP)；1-2 µg/mL(IHC (P))；2 µg/mL(IP)；0.5-1.0 µg/mL(WB)

产品详细信息MA5-14067 targets p53 in IHC (P), IP, and WB applications and shows reactivity with Human samples.The MA5-14067 immunogen is recombinant human p53 protein.靶标信息The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

数据：

p53 Antibody (MA5-14067) in IHC (P)Immunohistochemistry analysis of p53 showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a p53 Mouse Monoclonal Antibody (Product # MA5-14067) diluted in 3% BSA-PBS at a dilution of 1:50 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. p53 Antibody (MA5-14067) in ChIPEnrichment of endogenous p53 Protein at specific gene loci using Anti-p53 Protein Mouse Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-p53 Protein Mouse Monoclonal Antibody (Product # MA5-14067, 5 µg) on sheared chromatin from 2 million MCF-7 cells, UV treated for 30 minutes using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoter of active CDKN1A, CMBL, HES2, MKN2 gene, used as positive control target, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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参考文献：

1. Turkish neurosurgeryThe Correlation Between 1p/19q Codeletion, IDH1 Mutation, p53 Overexpression and Their Prognostic Roles in 41 Turkish Anaplastic Oligodendroglioma Patients.2. Cancer geneticsBCL2, BCL6, IGH, TP53, and MYC protein expression and gene rearrangements as prognostic markers in diffuse large B-cell lymphoma: a study of 44 Turkish patients.3. Applied immunohistochemistry and molecular morphology : AIMMHigh Coexpression of Runt-related Transcription Factor 2 (RUNX2) and p53 Independently Predicts Early Tumor Recurrence in Bladder Urothelial Carcinoma Patients.4. Neurochemical researchDifferential Expression of Adenosine P1 Receptor ADORA1 and ADORA2A Associated with Glioma Development and Tumor-Associated Epilepsy.5. Breast cancer research and treatmentRetrospective analysis of metastatic behaviour of breast cancer subtypes.

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