Progesterone Receptor Monoclonal Antibody (PR-AT 4.14)/孕激素受体单克隆抗体（PR-AT 4.14）

货号：MA1-410

规格：100 µg

价格：5468

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to residues G(533)

物种：其它

宿主：小鼠

抗体亚型：其它

克隆号：PR-AT 4.14

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	Assay Dependent(ChIP)；1/500(IF)；5 µg/mL(IHC (F))；5 µg/mL(IHC (P))；Assay dependent(IP)；1:200-1:1000(WB)

产品详细信息MA1-410 detects the progesterone receptor (PR) from human, non-human primate, bovine, rat, rabbit, and mouse tissues. This antibody does not cross-react with androgen receptor, estrogen receptor or glucocorticoid receptor.MA1-410 has been successfully used in Western blot, immunohistochemical and immunoprecipitation procedures. By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from T47D whole cell lysate.MA1-410 performs better by western blot using BSA as the blocking agent and in antibody diluent.The MA1-410 immunogen is a synthetic peptide corresponding to the binding domain residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor.MA1-410 can be used with blocking peptide PEP-016.靶标信息The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of theendometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

数据：

Progesterone Receptor Antibody (MA1-410) in IHCImmunohistochemistry was performed on normal deparaffinized Human uterus tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Progesterone Receptor (Product # MA1-410) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. Progesterone Receptor Antibody (MA1-410)PloS one 2011 -Figure 1 Progesterone Receptor expression in mesenchymal cells in the intestine, not in the epithelium. A-E) PR immunohistochemistry on the mouse colon (A) and small intestine (B,C) where rare cells express PR (arrowhead). And in an adenoma of an Apc Min/+ mouse (D). PR is widely expressed in the mouse uterus (E). F,G) In situ hybridization in mouse uterus (F) and small intestine (G). All murine tissue shown was taken from A female animal in diestrous stage, when progesterone is high [39] . H-J) PR Expression in the human colon is located in mesenchymal cells (I) and the smooth muscle layer (J), similar to the mouse intestine. For in situ hybridization, thick (10 um) sections were used, which makes identification of mesenchymal cells difficult. K) PR immunoblot on the mouse colon and small intestine. L) PR immunoblot on a panel of colon cancer cell lines shows no expression of either PR-A or PR-B isoform. The breast cancer cell line T47D is used as a positive control.

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参考文献：

1. Breast cancer research and treatmentVitamin D and androgen receptor-targeted therapy for triple-negative breast cancer.2. PloS oneProgesterone receptor expression declines in the guinea pig uterus during functional progesterone withdrawal and in response to prostaglandins.3. NeuroendocrinologyProgesterone receptor isoform expression in response to in utero growth restriction in the fetal guinea pig brain.4. Acta oto-laryngologicaNo direct nuclear effect of progesterone in the inner ear: other possible pathways.5. EndocrinologySubfertility linked to combined luteal insufficiency and uterine progesterone resistance.

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