RBPJ Polyclonal Antibody/RBPJ多克隆抗体

货号：720219

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Protein corresponding to Human RBPJ (aa 1-200)

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1.5 µg x 10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.This antibody is predicted to react with Mouse, Monkey, Bovine, Equine and Pig靶标信息CSL plays a role in the regulatory network through which muscle cells coordinate their structural and functional states during growth, adaptation, and repair. The protein is abundantly expressed in heart and skeletal muscle and expression is increased by passive stretch. Mutations in this gene can lead to X-linked deafness.

数据：

RBPJ Antibody (720219) in IFFor immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous CSL/ RBP J using CSL/RBP J Rabbit Polyclonal Antibody (Product # 720219, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of CSL/ RBP J protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c demonstrating predominant nuclear localization of CSL/ RBP J. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. RBPJ Antibody (720219) in ChIPEnrichment of endogenous CSL/RBP J protein at specific gene loci using Anti-CSL/RBP J Rabbit Polyclonal Antibody: Chromatin Immunoprecipiation (ChIP) was performed using Anti-CSL/RBP J Rabbit Polyclonal Antibody (Product # 720219, 3 µg) on sheared chromatin from 2 million HeLa cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active HES1, HES5 promoter region used as positive control target genes, and the region of the inactive HES5 upstream, SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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