RXRA Monoclonal Antibody (K8508)/RXRA单克隆抗体（K8508）

货号：433900

规格：100 µg

价格：6869

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Produced in BALB/c mouse ascites after inoculation

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

克隆号：K8508

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	Assay Dependent(ChIP)；0.1 µg/mL(ELISA)；Assay Dependent(GS)；5 µg/mL(ICC)；5 µg/mL(IF)；10-20 µg/mL(IHC)；Assay Dependent(IP)；2 µg/mL(WB)

产品详细信息This antibody specifically recognizes human RXR alpha and cross reacts with mouse and rat RXR alpha. This antibody does not recognize human RXR beta and gamma.靶标信息Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells. The Retinoid X Receptors (RXRs) family (RXR alpha, beta, and gamma), preferentially bind 9-cis -RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer. RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors.

数据：

RXRA Antibody (433900) in IFImmunofluorescence analysis of RXRA was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RXRA Monoclonal Antibody (Product # 433900) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. RXRA Antibody (433900) in WBKnockdown of RXRA was achieved by transfecting MCF7 cells with RXRA specific siRNAs (Silencer® select Product # s12385, s12386). Western blot analysis (Fig. a) was performed using nuclear enriched extracts from the RXRA knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with RXRA Monoclonal Antibody (Product # 433900, 1 µg/mL dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RXRA.

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