HIST2H2AB Polyclonal Antibody/HIST2H2AB多克隆抗体

货号：PA5-48625

规格：400 µL

价格：4806

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：KLH conjugated synthetic peptide between 1-30 amin

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	Lot-Specific	用法：	1.5 µg/1x10^6 cells(ChIP)；1:500(WB)

产品详细信息Predicted to react with rat, mouse, bovine and chicken based on sequence homology.靶标信息Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

数据：

HIST2H2AB Antibody (PA5-48625) in WBWestern blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), MDA-MB-231 (Lane 3), COS-7 (Lane 4) and A549 (Lane 5). The blot was probed with Anti-HIST2H2AB Polyclonal Antibody (Product # PA5-48625, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 14 kDa band corresponding to HIST2H2AB was observed across the cell lines tested. HIST2H2AB Antibody (PA5-48625) in ChIPEnrichment of endogenous H3.3 protein at specific gene loci using Anti-H3.3 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H3.3 Monoclonal Antibody (Product # MA5-24667, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs for the GAPDH PROMOTER, GAPDH EXON2 used as positive and MYOD, SAT2 satellite repeats, SAT Alpha used as negative target genes/binding sites. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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技术参数

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