Histone 1F0 Polyclonal Antibody/组蛋白1F0多克隆抗体

货号：PA5-38570

规格：100 µg

价格：0

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：A synthetic peptide derived from the internal regi

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	1 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；1:500(WB)

靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

数据：

Histone 1F0 Antibody (PA5-38570) in WBWestern blot analysis was performed on acid extracts (20 µg lysate) of PC-12 (Lane 1), PC-12 treated with Retinoic acid (100 uM for 12 days) (Lane 2), HEK-293 (Lane 3), MDA-MB-231 (Lane 4) and DU-145 (Lane 5). The blot was probed with Anti-Histone 1F0 Polyclonal Antibody (Product # PA5-38570, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 30 kDa band corresponding to Histone 1F0 was observed across the cell lines tested and enhanced upon differentiation with retinoic acid. Histone 1F0 Antibody (PA5-38570) in ChIPEnrichment of endogenous Histone H2B protein at specific gene loci using Anti-Histone H2B Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H2B Polyclonal Antibody (Product # PA1-41058, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the GAPDH gene (active) and MYOD, SAT2 satellite repeats, SAT Alpha (inactive). A schematic diagram of the GAPDH gene is shown on top of the figure. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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技术参数

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