H2AK119ub Polyclonal Antibody/H2AK119ub多克隆抗体

货号：720148

规格：100 µg

价格：0

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptide corresponding to Human HIST1H2AA (aa 116-1

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-2 µg x 10^6 cells(ChIP)；0.15-10 µg/mL(ELISA)；2-4 µg/1x10^6 cells(Flow)；2 µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Pig and Cat.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

H2AK119ub Antibody (720148) in IFImmunofluorescence was performed on fixed and permeabilized HeLa cells for detection of H2A-Ub using H2A-Ub Rabbit Polyclonal Antibody (Product # 720148, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of H2A-Ub protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of H2A-Ub. Panel e) represents control cells with no primary antibody to assess background. H2AK119ub Antibody (720148) in ELISACross-reactivity ELISA for Anti-Histone H2A-Ub Rabbit Polyclonal Antibody (Product # 720148) was performed against H2A-Ub, H2A-Pep1, H2A-Pep2, H2Ax and H2B-Ub by coating respective antigens at 20 ng/mL. Antibodies at 10, 2.5, 0.625 and 0.156 µg/mL were added and detected using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). The plate was developed using Stabilized Chromogen, TMB (Product # SB02).

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技术参数

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