Histone 1F0 Polyclonal Antibody/组蛋白1F0多克隆抗体

货号：PA5-51466

规格：100 µL

价格：5902

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Recombinant protein corresponding to Human Histone

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.05 mg/mL	用法：	Assay Dependent(ChIP)；0.25-2 µg/mL(ICC)；0.25-2 µg/mL(IF)；1:200-1:500(IHC)；0.04-0.4 µg/mL(WB)

产品详细信息Immunogen sequence: MTENSTDHPK YSDMIVAAIQ AEKNRAGSSR QSIQKYIKSH YKVGENADSQ IKLSIKRLVT TGVLKQTKGV GASGSFRLAK SDEPKKSVAF KKTKKEIKKV ATPKKASKHighest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 39%.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

数据：

Histone 1F0 Antibody (PA5-51466) in IFImmunofluorescent staining of Histone 1F0 in human cell line A-431 using a Histone 1F0 Polyclonal Antibody (Product # PA5-51466) shows localization to nucleus and actin filaments. Histone 1F0 Antibody (PA5-51466) in IFImmunofluorescence analysis of Histone 1F0 was performed using 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Histone 1F0 Rabbit Polyclonal Antibody (Product # PA5-51466) at 5 microgram/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-46371.htm