CD335 (NKp46) Monoclonal Antibody (29A1.4), eFluor 660,

货号：50-3351-82

规格：100 µg

市场价格：2580

产品类型：流式抗体

品牌：eBioscience

抗原：CD335 (NKp46)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：29A1.4

荧光染料：eFluor 660

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660
浓度：	0.2 mg/mL	用法：	1 µg/test(Flow)

产品详细信息Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

数据

CD335 (NKp46) Antibody (50-3351-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD49b (Integrin alpha 2) PE (Product # 12-5971-82) and 0.5 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.5 µg of Anti-Mouse CD335 (NKp46) eFluor® 660 (right). Total viable cells were used for analysis.

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参考文献

1. Cellular & molecular immunologyThe breast tumor microenvironment alters the phenotype and function of natural killer cells.2. Journal of immunology (Baltimore, Md. : 1950)Neonatal rhinovirus infection induces mucous metaplasia and airways hyperresponsiveness.3. Journal of immunology (Baltimore, Md. : 1950)Diverse roles for T-bet in the effector responses required for resistance to infection.4. Nature immunologyIL-22 bridges the lymphotoxin pathway with the maintenance of colonic lymphoid structures during infection with Citrobacter rodentium.5. Wellcome open researchConventional NK cells and ILC1 are partially ablated in the livers of Ncr1iCreTbx21fl/flmice.

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