H3K18ac Recombinant Rabbit Monoclonal Antibody (RM166)/H3K18ac重组兔单克隆抗体（RM166）

货号：MA5-24669

规格：100 µg

价格：4130

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Acetyl-peptide corresponding to the Acetyl-Histone

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：RM166

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	0.2-1 µg/mL(ELISA)；5 µg/mL(ICC)；5 µg/mL(IF)；1-10 µg/mL(IHC)；0.5 µg/mL- 2 µg/mL(WB)；0.25 µg/mL(Array)

产品详细信息This antibody reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K18ac Antibody (MA5-24669) in IFImmunofluorescence analysis of Acetyl-Histone H3 (Lys18) Monoclonal Antibody (RM166) was performed using 70% confluent log phase HeLa cells treated with sodium butyrate. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H3 (Lys18) Monoclonal Antibody (RM166) (MA5-24669) at 5 µg/mL in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the untreated cells with relatively lower expression of Acetyl-Histone H3 (Lys18) Monoclonal Antibody (RM166). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K18ac Antibody (MA5-24669) in ELISASandwich ELISA against acetylated histone H3 at Lys 18 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (1 mg/mL) as the capture antibody and Acetyl-Histone H3 (Lys18) Antibody (Product # MA5-24669) 2 mg/mL as the detection antibody.

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