H3K27ac Polyclonal Antibody/H3K27ac多克隆抗体

货号：720096

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Acetylated peptide (Lys27) corresponding to Human

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 µg/mL(ICC)；0.5 µg/mL(IF)；1-2 µg/mL(WB)；0.25 µg/mL(Array)

产品详细信息These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.720096 was used in western blot to successfully detect H3K27ac in acid extracted histones from human cells.Since it is conserved across species, the antibody may react with many other species.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K27ac Antibody (720096) in IFImmunofluorescence was performed on fixed and permeabilized HepG2 cells for detection of Histone H3K27ac using Anti-Histone H3K27ac Rabbit Polyclonal Antibody (Product # 720096, 0.5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Histone H3K27ac protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938,). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Histone H3K27ac. Panel e) represents control cells with no primary Antibody to assess background. H3K27ac Antibody (720096) in WBWestern blot analysis was performed on acid cell extracts (30 µg lysate) of HCT116 (Lane1), HCT116 treated with SAHA (Lane 2), NIH/3T3 (Lane 3), NIH/3T3 treated with SAHA (Lane 4), HEK-293 (Lane 5), HEK-293 treated with SAHA (Lane 6), HT-29 (Lane 7), HT-29 treated with SAHA (Lane 8), K562 (Lane 9), K562 treated with SAHA (0.5 uM/16 hours) (Lane 10). The blots were probed with Anti-Histone H3K27ac Rabbit Polyclonal Antibody (Product # 720096, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 17kDa band corresponding to Histone H3K27ac was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1. Toxicology and applied pharmacologySelective inhibition of CTCF binding by iAs directs TET-mediated reprogramming of 5-hydroxymethylation patterns in iAs-transformed cells.

技术参数

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