H3K23me2 Recombinant Rabbit Monoclonal Antibody (RM171)/H3K23me2重组兔单克隆抗体（RM171）

货号：MA5-24678

规格：100 µg

价格：4130

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Di-methyl-peptide corresponding to Di-methyl-Histo

物种：人

宿主：兔

抗体亚型：IgG

克隆号：RM171

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；0.1-0.5 µg/mL(ELISA)；5 µg/mL(ICC)；5 µg/mL(IF)；1 µg/mL(WB)；0.1-0.5 µg/mL(Array)

产品详细信息This antibody reacts to Histone H3 dimethylated at Lysine 23 (K23me2). No cross reactivity with monomethylated Lysine 23 (K23me1) or trimethylated Lysine 23 (K23me3), or other methylation in histone H3.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K23me2 Antibody (MA5-24678) in IFImmunofluorescence analysis of Di-Methyl-Histone H3 (Lys23)was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Monoclonal Antibody (Product # MA5-24678) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Project # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K23me2 Antibody (MA5-24678) in ChIPEnrichment of endogenous Di-Methyl-Histone H3 (Lys23) protein at specific gene loci using Anti- Di-Methyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Di-Methyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody (Product # MA5-24678, 3 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs for the promoters of c-FOS, GAPDH used as positive, and MYOD, SAT2 satellite repeats used as negative target genes/binding sites. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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