H3K9me2 Polyclonal Antibody/H3K9me2多克隆抗体

货号：PA5-16195

规格：100 µL

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the amino termi

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：		用法：	1:250(ICC)；1:250(IF)；1:25(IP)；1:1000(WB)

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with di-methylated or tri-methylated histone H3 Lys27.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K9me2 Antibody (PA5-16195) in IFImmunofluorescence analysis of Di-Methyl-Histone H3 (Lys9)was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Polyclonal Antibody (Product # PA5-16195) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K9me2 Antibody (PA5-16195) in WBWestern blot analysis was performed on HeLa (Lane 1), HCT 116 (Lane 2), MDA-MB-231 (Lane 3), MCF7 (Lane 4) and JURKAT (Lane 5). The blot was probed with Anti- Di-Methyl-Histone H3 (Lys9) (Product #PA5-16195, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Di-Methyl-Histone H3 (Lys9) was observed across the cell lines tested.

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参考文献：

1. Nature communicationsThe cell-wide web coordinates cellular processes by directing site-specific Ca2+flux across cytoplasmic nanocourses.

技术参数

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