H3K36me3 Recombinant Rabbit Monoclonal Antibody (RM155), ChIP-Verified/H3K36me3重组兔单克隆抗体（RM155），ChIP验证

货号：MA5-24687,MA5-24687-20UG

规格：100 µg,20 µg

价格：4130,1386

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Tri-methyl-peptide corresponding to tri-methyl-His

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：RM155

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1-5 µg(ChIP)；0.2-1 µg/mL(ELISA)；5 µg/mL(ICC)；5 µg/mL(IF)；0.5 µg/mL-2 µg/mL(WB)；0.25 µg/mL(Array)

产品详细信息This antibody reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in histone H3.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K36me3 Antibody (MA5-24687) in IFImmunofluorescence analysis of Tri-Methyl-Histone H3 (Lys36) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tri-Methyl-Histone H3(Lys36) Rabbit Monoclonal Antibody(RM155) (Product # MA5-24687) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K36me3 Antibody (MA5-24687) in ChIPK-MetStat Panel, SNAP-ChIP™ Spike-in (Product # A47356), a proprietary technology developed byEpiCypher™was used to analyze the performance of H3K36me3 antibody (Product # MA5-24687) in ChIP. SNAP-ChIP panels consist of a pool of DNA-barcoded recombinant nucleosomes harboring histone PTMs that are spiked-in to a ChIP reaction to assess efficiency and specificity of the antibody. The K-MetStat panel includes an unmodified control plus nucleosomes containing histones with mono, di and tri-methyl forms of lysine residues on histone H3 and H4 as shown on the x-axis. Recovery of each unique DNA-barcoded nucleosome is quantified to determine how much of each PTM is immunoprecipitated in the ChIP reaction (for more information seereference). H3K36me3 antibody was tested in native ChIP with 3 µg HEK-293 cell chromatin and 3 µg antibody. Specificity (left Y-axis) was determined by qPCR to each modified nucleosome in the panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded nucleosome target immunoprecipitated relative to Input. All bars represent mean ± SEM.

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