H3K4me3 Monoclonal Antibody (G.532.8)/H3K4me3单克隆抗体（G.532.8）

货号：MA5-11199

规格：100 µL

价格：4692

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the amino termi

物种：其它

宿主：兔

抗体亚型：IgG

克隆号：G.532.8

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1:400(ICC)；1:400(IF)；1:200(IHC (P))；1:1000(WB)；1:2,000(Array)

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with methylated histone H3 Lys9, Lys27, Lys36 or methylated histone H4 Lys20.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K4me3 Antibody (MA5-11199) in IFImmunofluorescence analysis of Tri-Methyl-Histone H3 (Lys4) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Monoclonal Antibody (Product # MA5-11199) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K4me3 Antibody (MA5-11199) in IHCImmunohistochemical analysis using a Tri-Methyl-Histone H3 (Lys4) monoclonal antibody (Product # MA5-11199).

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参考文献：

1. The Journal of biological chemistryIdentification of mixed lineage leukemia 1(MLL1) protein as a coactivator of heat shock factor 1(HSF1) protein in response to heat shock protein 90 (HSP90) inhibition.

技术参数

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