Phospho-c-Fos (Thr232) Polyclonal Antibody/Phospho-c-Fos（Thr232）多克隆抗体

货号：44-280G

规格：100 µL

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：The antiserum was produced against a chemically sy

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：		用法：	1-3 µg x 10^6 cells(ChIP)；1:250(ICC)；1:250(IF)；1-2 µg/mL(WB)；1:20(IHC (P))；1:1000(WB)

靶标信息FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated byvarious stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.

数据：

Phospho-c-Fos (Thr232) Antibody (44-280G) in IFImmunofluorescence analysis of Phospho-c-Fos pThr232 was done on 70% confluent log phase HeLa cells treated with 20 ng of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Phospho-c-Fos pThr232 Rabbit Polyclonal Antibody (Product # 44-280G) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. Phospho-c-Fos (Thr232) Antibody (44-280G) in IHC (P)Immunohistochemistry analysis of Phospho-c-Fos pThr232 showing staining in the nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Phospho-c-Fos pThr232 Polyclonal Antibody (Product # 44-280G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

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参考文献：

1. Molecular and cellular biologyDown-regulation of c-Fos/c-Jun AP-1 dimer activity by sumoylation.

技术参数

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