H4K12ac Polyclonal Antibody/H4K12ac多克隆抗体

货号：PA5-17773

规格：100 µL

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic acetylated peptide corresponding to resi

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：		用法：	1:250(ICC)；1:250(IF)；1:1000(WB)；1:2000(Array)

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with other acetylated histones.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

H4K12ac Antibody (PA5-17773) in IFImmunofluorescence analysis of Acetyl-Histone H4 (Lys12) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H4 (Lys12) Rabbit Polyclonal Antibody (Product # PA5-17773) at 1:250 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. H4K12ac Antibody (PA5-17773) in IFImmunofluorescent analysis of Acetyl-Histone H4 (Lys12) in HeLa cells, TSA-treated, using an Acetyl-Histone H4 (Lys12) polyclonal antibody (Product # PA5-17773) (green). Actin filaments are labeled with a fluorescent red phalloidin.

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