STAT3 Monoclonal Antibody (9D8)/STAT3单克隆抗体（9D8）

货号：MA1-13042

规格：100 µL

价格：5467

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Residues 655-770 from human recombinant protein ex

物种：其它

宿主：小鼠

抗体亚型：其它

克隆号：9D8

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1-3 µL(ChIP)；1:100(ICC)；1:100(IF)；1:1600(IHC (P))；2 µg(IP)；1:5000(WB)

产品详细信息MA1-13042 detects STAT3 from human, mouse, monkey and rat samples.MA1-13042 has been successfully used in Western blot, ChIP, immunofluorescence, immunohistochemistry (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a 88 kDa proteincorresponding to STAT3. Positive control: HepG2 cells.The MA1-13042 antigen is a recombinant protein corresponding to residues 655-770 of human STAT3.Antibodies to this protein (and modification) werepreviously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.靶标信息STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

数据：

STAT3 Antibody (MA1-13042) in IFImmunofluorescent analysis of STAT3 using anti-STAT3 (9D8) monoclonal antibody (Product # MA1-13042) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing STAT3 (Product # MA1-13042), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product # 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. STAT3 Antibody (MA1-13042) in IHCImmunohistochemistry was performed on biopsies of normal and cancer tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product # 31873) for 20 minutes at room temperature. Tissues were then probed at a dilution of 1:1600 with a mouse monoclonal antibody recognizing STAT3 (Product # MA1-13042) overnight at 4°C in a humidified chamber. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 400X magnification. Results demonstrate both nuclear and cytoplasmic localization of STAT3.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1. International journal of oncologyEffects of cell-cell crosstalk on gene expression patterns in a cell model of renal cell carcinoma lung metastasis.2. PloS oneAn Autocrine Cytokine/JAK/STAT-Signaling Induces Kynurenine Synthesis in Multidrug Resistant Human Cancer Cells.3. Cell death and differentiationFGF2 regulates melanocytes viability through the STAT3-transactivated PAX3 transcription.4. BloodWhole-genome sequencing reveals oncogenic mutations in mycosis fungoides.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-46544.htm