CBP Polyclonal Antibody

货号：PA1-847

规格：100 µg

价格：5468

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to residues A(162)

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1-3 µL(ChIP) ; 1:50-1:500(ICC);1:50-1:500(IF);1:1000-1:5000(IHC(P)); 1-2 µg/mL(WB)

产品详细信息PA1-847 detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) from human and mouse tissues and cells. PA1-847 has been successfully used in ChIP, ICC/IF, Western blot and IHC-P procedures. By Western blot, this antibody detects an ~265 kDa protein representing CBP and a larger, unknown protein at ~400 kDa from HeLa cell lysate.The PA1-847 immunogen is a synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP. The PA1-847 immunizing peptide (Cat. # PEP-052) is available for use in neutralization and control experiments. 靶标信息CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.

数据：

CBP Antibody (PA1-847) in IF Immunofluorescent analysis of CREB Binding Protein (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB Binding Protein polyclonal antibody (Product # PA1-847) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x. CBP Antibody (PA1-847) in IHC (P) Immunohistochemistry analysis of CREB Binding Protein showing staining in the cytoplasm and nucleus of paraffin-embedded mouse colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CREB Binding Protein polyclonal antibody (Product # PA1-847) diluted in 3% BSA-PBS at a dilution of 1:2000 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

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