CBP Polyclonal Antibody

货号：PA5-27369

规格：100 µL

价格：5935

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant fragment corresponding to a region wit

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.44 mg/mL	用法：	1:100-1:1000(ICC) ; 1:100-1:1000(IF);1:100-1:1000(IHC(P));1:100-1:500(IP); 1:500-1:3000(WB)

产品详细信息Recommended positive controls: 293T, A431, HeLa, HepG2, A549, H1299, HCT-116, Vero E6.Predicted reactivity: Mouse (94%), Rat (94%), Rhesus Monkey (100%), Bovine (93%).Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. 靶标信息CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.

数据：

CBP Antibody (PA5-27369) in IF Immunofluorescence analysis of CBP was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CBP Polyclonal Antibody (Product # PA5-27369) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. CBP Antibody (PA5-27369) in IHC (P)Immunohistochemical analysis of CBP in the nucleus of paraffin-embedded human cervical carcinoma using a CBP polyclonal antibody (Product # PA5-27369) at a dilution of 1:750.

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技术参数

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