F555L Thermo DNA聚合酶,Phusion U Hot Start DNA Polymerase/Phusion U热启动DNA聚合酶-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F555L Thermo DNA聚合酶,Phusion U Hot Start DNA Polymerase/Phusion U热启动DNA聚合酶

2024-10-24

Phusion U Hot Start DNA Polymerase/Phusion U热启动DNA聚合酶

货号：F555L,F555S

规格：500 units,100 units

价格：5014,1276

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.Phusion U Hot Start DNA Polymerase is also available in the Master Mix and Green formats. Phusion U Hot Start PCR Master Mix (F-533S and F-533L) is convenient 2X mix designed to minimize the number of pipetting steps. Only template and primers need to be added by the user. Phusion U Green format (F-556S and F-556L) is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

优点：

-Accuracy—high fidelity DNA polymerase (25x Taq)-Uracil-tolerance—engineered to incorporate dUTP and amplify uracil containing templates-Specificity—hot start for reduced nonspecific amplification and primer degradation-Speed—short extension times (15-30 s/kb)

数据：

图1.Incorporates dUTP into PCR products2 kb fragment from 50 ng human genomic DNA amplified in the presence of 0.2 mM dUTP according to recommendations of different suppliers of proofreaders with dUTP incorporation ability.

图2.Highest yields and specificity with bisulphite-converted DNAFive proofreading DNA polymerases and hot startTaqpolymerase were used to amplify 798 bp fragment of bisulphite-converted human genomic DNA. Phusion U Hot Start DNA Polymerase provided high yields of specific products whereas all other enzymes delivered zero or lower yields, with some also amplifying non-specific products.

组成成分：

▪Phusion U Hot Start DNA Polymerase, 2 U/µL▪ 5X Phusion HF Buffer▪ 5X Phusion GC Buffer▪ DMSOBoth Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C

F555L Thermo DNA聚合酶,Phusion U Hot Start DNA Polymerase/Phusion U热启动DNA聚合酶

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