ATF2 Polyclonal Antibody

货号：PA5-78831

规格：100 µg

价格：2746

产品类型：标签抗体

品牌：Thermo Fisher

抗原：E.coli-derived human ATF2 recombinant protein (Pos

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：其它

荧光染料：其它

类型:	一抗	同型对照：
浓度：	500μg/mL	用法：	1-3 µg/1x10^6 cells(Flow);0.5-1 µg/mL(ICC)；0.5-1 µg/mL(IF);0.5-1 µg/mL(IHC(F));0.5-1 µg/mL(IHC(P));0.1-0.5 µg/mL(WB)

产品详细信息Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL. 靶标信息The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.

数据：

ATF2 Antibody (PA5-78831) in IHC (P) Immunohistochemistry analysis of ATF2 on paraffin-embedded human intestinal cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with ATF2 polyclonal antibody (Product# PA5-78831) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method. ATF2 Antibody (PA5-78831) in WB Western blot analysis of ATF2 in Lane 1: recombinant human ATF2 protein using 0.5 ng per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with ATF2 polyclonal antibody (Product # PA5-78831) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

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