Suggested Application

Buffer

Activity

Reference Sample

20mM Tris-HCl (pH8.0)

100%

Bacterial Genomic DNA Isolation

10mM Tris-HCl, 1mM EDTA, 0.5% SDS (pH8.0)

108%

Mammalian Genomic DNA Isolation from Tissue

10mM Tris-HCl, 25mM EDTA, 100mM NaCl, 1% SDS (pH8.0)

171%

Plant Genomic DNA Isolation from Tissue

100mM Tris-HCl, 100mM EDTA, 250mM NaCl, 1% Sarkosyl (pH8.0)

118%

Inactivation of Calf Intestinal Alkaline Phosphatase

10mM Tris-HCl, 5mM EDTA, 50mM NaCl, 1mM DTT, 0.5% SDS (pH7.9)

104%

Denaturation of Proteins

50mM Tris-HCl, 1mM CaCl2, 3mM DTT, 2M Urea (pH8.0)

66%

PCR Buffer

10mM Tris-HCl, 1.5mM MgCl2, 0.1% Triton®X-100 (pH8.8)

158%