GCN5 Monoclonal Antibody (S.775.4)

货号：MA5-14884

规格：100 µL

价格：4880

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the amino termi

物种：其它

宿主：兔

抗体亚型：IgG

克隆号：S.775.4

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1:100(ICC);1:100(IF)；1:200（IP）;1:500(WB)

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with the related PCAF protein. 靶标信息SAGA (Spt-Ada-Gen5 histone acetyltransferase complex) is a histoneacetylase complex which has Gcn5p as catalyst subunit and functions overlapping with the fundamental transcription factor TFIID which has Taf1p as catalyst subunit. However, SAGA and TFIID have different allotment and each accomplishes the important role in the transcription for Housekeeping gene group and Stress Responding gene group. Also, SAGA is a gigantic protein complex which is composed of Ada protein group (5 kinds), TBP related protein group (4 kinds), TAF protein group (5 kinds that also pertain to TFIID), and other protein groups (>6 kinds). As its molecular function, there is the chemical modification of histone, or the recruitment by direct interaction of transcription regulating factor on DNA, or the control of transcription starting reaction by TBP. Gcn5p that show histoneacetylase activity is one kind of the above-mentioned Ada protein group, and in the case of budding yeast it is composed of 439 amino acid residues.

数据：

GCN5 Antibody (MA5-14884) in IF Immunofluorescent analysis of GCN5L2 in HeLa cells using a GCN5L2 monoclonal antibody (Product # MA5-14884) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye. GCN5 Antibody (MA5-14884) in WB Knockdown of GCN5 was achieved by transfecting HeLa cells with GCN5 specific siRNAs (Silencer® select Product # s5657). Western blot analysis (Fig. a) was performed using whole cell extracts from the GCN5 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with GCN5 Monoclonal Antibody (Product # MA5-14884, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to GCN5.

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