Thermo Alexa Fluor 555 蛋白标记,APEX Alexa Fluor 555 Antibody Labeling Kit/Alexa Fluor 555抗体标记试剂盒

2024-10-24

APEX Alexa Fluor 555 Antibody Labeling Kit/Alexa Fluor 555抗体标记试剂盒

货号:A10470

规格:1 kit

价格:0

产品类型:蛋白标记

品牌:Thermo Fisher

The APEX Antibody Labeling Kits are our best option for covalently attaching a fluorophore to small amounts of IgG antibody (~10–20 μg). It is ideal for the efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Labeled antibodies are ready for use in imaging or flow cytometry applications in as little as 2.5 hours with very little hands on time.Better Results and Workflows With Primary Labeled AntibodiesA primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Further, multiple primary antibodies of the same isotype or derived from the same species can easily be used in the same experiment if they are directly labeled with compatible fluorophores.Contaminating Proteins or Protein Stabilizers Are Not a ProblemMany IgG antibodies are often available only in small quantities and packaged with stabilizing proteins, such as BSA, or other contaminants which can interfere with the amine-reactive labeling reagents. The APEX Antibody Labeling Kits avoids this by utilizing a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX antibody labeling tip. Contaminants are simply eluted through the tip, prior to applying the amine-reactive label.
参考文献:
1.Low-density Lipoprotein Receptor-related Protein-1 (LRP1) Mediates Autophagy and Apoptosis Caused by Helicobacter pylori VacA.2.Polydom/SVEP1 is a ligand for integrin a9ß1.

技术参数

产品优点 • Labeled antibodies typically ready to use in 2.5 hours (~15 minutes hands on time)• Designed to label 10–20 μg of IgG• Covalent attachment• Compatible with contaminating proteins or stabilizers like BSA• No columns needed; everything you need is supplied for 5 separate labelings• Choose from Alexa Fluor® 488, 555, 568, 594, and 647 dyes, Oregon Green® 488 dye, Pacific Blue™ dye, and Biotin-XX.

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