Aurora B Polyclonal Antibody

货号：36-5200

规格：100 µg

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide derived from the N-terminal regi

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.25 mg/mL	用法：	1 µg/mL(ICC);1 µg/mL(IF);1 µg/mL(WB)

靶标信息Aurora-B kinase (AurB) is a serine/threonine kinase which is the enzymatic core of the Chromosomal Passenger Complex (CPC). This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

数据：

Aurora B Antibody (36-5200) in IF Immunofluorescent analysis of Aurora B Antibody was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Aurora B Antibody (Product # 36-5200) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification. Aurora B Antibody (36-5200) in WB Western blot was performed using Anti-Aurora B Polyclonal Antibody, (Product # 36-5200) and a 39 kDa band corresponding to Aurora B was observed across cell lines tested except in T47D. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with Nocodazole (200ng/ml for 24hr) (Lane 2), NTera (Lane 3), A549 (Lane 4), T47D (Lane 5), THP1 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). An uncharacterized band (*) was observed at ~78kDa across the samples tested.

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技术参数

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