Interferon alpha (Cell culture) Human ELISA Kit ELISA试剂盒,Interferon alpha (Cell culture) Human ELISA Kit/人干扰素α（细胞培养）ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Interferon alpha (Cell culture) Human ELISA Kit ELISA试剂盒,Interferon alpha (Cell culture) Human ELISA Kit/人干扰素α（细胞培养）ELISA试剂盒

2024-10-24

Interferon alpha (Cell culture) Human ELISA Kit/人干扰素α（细胞培养）ELISA试剂盒

货号：411001,411002

规格：96 Tests,5X96 Tests

价格：7875,29550

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

检测范围	12.5-500 pg/ml or 156-5000 pg/ml
样本类型/体积	上清液 100 μL
操作时间	1 hr 20 min
测试时间	3 hr 15 min
Homogenous (no wash)	No
批间变异系数（CV）	<8%
批内变异系数（CV）	<8%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	5 x 96 Tests
组份	- 预包被96孔板 - 检测抗体浓缩液 - 抗-lgG-HRP浓缩液 - 标准品 - 洗涤浓缩液 - 底物溶液 - 样品稀释液 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IFN alpha
Protein aliases	alpha-2a interferon, IFN-alpha 1b, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFN-alpha-2, Interferon alpha-A, LeIF A, interferon alpha 1b, interferon alpha 2a, interferon alpha 2b, interferon alpha A, Interferon alpha-1/13, Interferon alpha-2, interferon, alpha 1, alpha 2, interferon-alpha1
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	IFL, IFN, IFN-ALPHA, IFN-alphaA, IFN-alphaD, IFNA, IFNA1, IFNA13, IFNA2, IFNA2A, IFNA2B, IFNA2C, IFNA@, INFA2
Gene ID	(Human) 3439,(Human) 3440
Gene symbol	IFNA1, IFNA2
UniProt ID	(Human) P01562,(Human) P01563

The Human Interferon alpha A, 2, A/D, D, K and 4b (Hu IFNA, IFN2, IFNA/D, IFND, IFNK and IFN4b ) ELISA quantitates Hu IFN in human cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFN.人干扰素αA，2，A / D，D，K和4b（Hu IFNA，IFN2，IFNA / D，IFND，IFNK和IFN4b）ELISA定量检测人细胞培养基中的IFN。该检测试剂盒将只识别天然和重组Hu IFN。Principle of the method 方法原理The Human IFN solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IFN固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准品或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二个（检测）抗体形成三明治，该第二个抗体与捕获抗体在不同表位上的靶标结合。已将结合酶掺入测定中。经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样本中存在的目标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.IFNα（干扰素α）是一种I型干扰素，属于脊椎动物专有的结构相关的物种特异性蛋白家族。 IFN-α与其受体组分的相互作用导致许多信号通路的活化，这些信号通路主要参与针对病毒感染的先天免疫应答。在人类中，IFNα的基因位于9号染色体上。I型干扰素由14种不同的α同工型（特异性稍有不同的亚型）以及单个β，ω，ε和κ同工型组成。同源分子存在于许多物种中，包括大鼠和小鼠（以及大多数哺乳动物），并且已经在鸟类，爬行动物和鱼类中得到鉴定。 IFNα主要由浆细胞样树突状细胞产生，是抗病毒先天免疫应答的有效成分，可调节适应性免疫。与IFNα功能障碍有关的疾病包括丙型肝炎和肝炎。For ResearchUse Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据

Interferon alpha (Cell culture) Human ELISA KitRepresentative standard curve for the high-sensitivity range of Human IFN-α ELISA

参考文献：

1.Stem cells and development

Mesenchymal stromal cells, colony-forming unit fibroblasts, from bone marrow of untreated advanced breast and lung cancer patients suppress fibroblast colony formation from healthy marrow.2.Molecular therapy : the journal of the American Society of Gene TherapyIntravesical Ad-IFNalpha causes marked regression of human bladder cancer growing orthotopically in nude mice and overcomes resistance to IFN-alpha protein.3.Journal of immunology (Baltimore, Md. : 1950)Plasmacytoid dendritic cells regulate Th cell responses through OX40 ligand and type I IFNs.4. Diabetes research and clinical practiceIn vitro mononuclear cell production of tumour necrosis factor-alpha and weight loss.

技术参数

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Interferon alpha (Cell culture) Human ELISA Kit ELISA试剂盒,Interferon alpha (Cell culture) Human ELISA Kit/人干扰素α（细胞培养）ELISA试剂盒

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