SIRT3 Polyclonal Antibody

货号：PA5-28402

规格：100 µL

价格：4880

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to a region within

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.7 mg/mL	用法：	1:100-1:1000(ICC);1:100-1:1000(IF);1:100-1:1000(IHC(P));1:500-1:3000(WB)

产品详细信息Recommended positive controls: HepG2.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.

数据：

SIRT3 Antibody (PA5-28402) in IFImmunofluorescence analysis of SIRT3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with ice-cold acetone for 5 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SIRT3 Polyclonal Antibody (Product # PA5-28402) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green).Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. SIRT3 Antibody (PA5-28402) in IHC (P)Immunohistochemical analysis of SIRT3 in paraffin-embedded human endometrial carcinoma using a polyclonal SIRT3 antibody, C-term (Product # PA5-28402) at a dilution of 1:500. SIRT3 Antibody (PA5-28402)Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with SIRT3 siRNA and reduction of signal was observed in Western Blot using SIRT3 Polyclonal Antibody (Product # PA5-28402).Knockdown validation info.

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