组蛋白乙酰化修饰,H3K4,多克隆抗体,HDAC1 Polyclonal Antibody

2024-10-24

HDAC1 Polyclonal Antibody

货号:49-1025

规格:50 µg

价格:4809

产品类型:标签抗体

品牌:Thermo Fisher

抗原:raised in rabbits against the C-terminal region of

物种:人/小鼠/大鼠

宿主:兔

抗体亚型:IgG

荧光染料:其它

类型:一抗同型对照:
浓度: 1.73 mg/mL用法:1/200 - 1/400(Flow) ;1:4000(ELISA);1:500(ICC);1:500(IF);1:1,000(WB);2 µg/4x10^6 cells(ChIP-Seq)
靶标信息Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.
数据:

HDAC1 Antibody (49-1025) in IFHeLa cells were stained with the anti-HDAC1 antibody (Product # 49-1025) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

HDAC1 Antibody (49-1025) in WBKnockdown of HDAC1 was achieved by transfecting MCF7 cells with HDAC1 specific siRNAs (Silencer® select Product # s73). Western blot analysis (Fig. a) was performed using Modified whole cell extracts (1% SDS) from the HDAC1 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Anti-HDAC1 Polyclonal Antibody (Product # 49-1025, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Densitometric analysis of this Western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to HDAC1..
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