Equine Resistin ELISA Kit ELISA试剂盒,Equine Resistin ELISA Kit/马抵抗素ELISA试剂盒

2024-10-24

Equine Resistin ELISA Kit/马抵抗素ELISA试剂盒

货号:EE7RB,EE7RBX5,EE7RBX10

规格:96 Tests,5X96 Tests,10X96 tests

价格:6641,29884,57271

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

46 pg/mL

检测范围46-3,000 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 Tests

组份

- 预包被96孔板

- 标准品

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameResistin

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Equine Resistin quantitates equine Resistin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant equine Resistin.马抵抗素定量测定血清,血浆,上清液中的马抵抗素。该检测试剂盒将只识别天然和重组马抗性素。Target information 靶标信息Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.脂肪组织是动物的能量储备,并且受到严格监管。脂肪细胞产生并分泌许多生理上重要的蛋白质,例如脂蛋白脂肪酶(LPL),瘦素,30 kDa的脂肪细胞补体相关蛋白(Acrp30),周脂蛋白和抵抗素。抵抗素,也称为脂肪细胞分泌因子(ADSF)和FIZZ3,是由脂肪细胞特异性分泌的细胞因子。虽然对抵抗素的功能尚不十分了解,但它似乎具有关键的旁分泌作用,并且在2型糖尿病中具有建议的作用。研究表明,在肥胖的糖尿病小鼠中,血清抵抗素水平升高,这对胰岛素具有拮抗作用。其他研究表明,抵抗素会引起小鼠胰岛素抵抗和葡萄糖不耐症。抵抗素基因表达需要C / EBP alpha,这对于表达是必要的和充分的。研究表明,已显示具有组蛋白乙酰转移酶(HAT)活性的共激活剂CBP和p300,在抵抗素启动子处将组蛋白充分乙酰化,表明它们在激活染色质以抵抗抵抗素转录方面发挥了作用。已显示许多PPARγ和RXR配体下调脂肪细胞中抵抗素的表达。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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