Cytokeratin Pan Antibody Cocktail

货号：MA5-13203

规格：500 µL

价格：3850

产品类型：标签抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG

克隆号：PAN-CK

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.2mg/mL	用法：	1:20-1:200（ICC）；1:20-1:200(IF);1-2 µg/mL（IHC(P)）；1:1000-1:5000(WB）

产品详细信息This antibody is a broad-spectrum pan-keratin monoclonal antibody cocktail which recognizes keratin 4, 5, 6, 7, 8, 10, 13, 14, 18 and 19. This product can differentiate between epithelial and non-epithelial tumors.For staining of formalin-fixed tissue,digest sections wtih trypsin at 1 mg/mL in PBS, 10 min at 37°C, or Protease XXV at 1 mg/mL in PBS for 5 min at 37°C.靶标信息Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

数据：

Cytokeratin Pan Antibody (MA5-13203) in IFImmunofluorescent analysis of Cytokeratin Pan (green) showing staining in the cytoplasm of MCF-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytokeratin Pan monoclonal antibody (Product # MA5-13203) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. Cytokeratin Pan Antibody (MA5-13203) in IHC (P)Immunohistochemistry analysis of Cytokeratin Pan showing positive staining in the cytoplasm of paraffin-treated Human colon carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cytokeratin Pan monoclonal antibody (Product # MA5-13203) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Cytokeratin Pan Antibody (MA5-13203) in WBWestern blot analysis of Cytokeratin Pan was performed by loading 25 µg of A431 (Lane 1), Hela (Lane 2), and PC12 cell lysates (Lane 3) and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4°C overnight. The membrane was probed with a Cytokeratin Pan monoclonal antibody (Product # MA5-13203) at a dilution of 1:2000 (A431 and Hela) and 1:1000 (PC12) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at 40-67 kDa in A431 and Hela cell lines.

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