HP1 beta Monoclonal Antibody (1MOD-1A9)

货号：MA3-053

规格：50 µL

价格：4693

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant HP1-beta mouse

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1MOD-1A9

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1:100（ChIP）;1:500(ELISA);1:250（ICC）；1:250(IF);1:2000(WB）

产品详细信息MA3-053 detects HP1-beta in human samples.MA3-055 has been succesfully used in western blot, immunofluorescence, ELISA and ChIP applications.Immunogen is a HP1-beta recombinant mouse靶标信息This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified.

数据：

HP1 beta Antibody (MA3-053) in IFImmunofluorescent analysis of HP1-beta (green) in HeLa cells. The cells were fixed with formalin for 15 minutes, permeabilized with 1X Permeablilization buffer (Product # 8408400) for 15 minutes, and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with HP1-beta monoclonal antibody (Product # MA3-053) at a dilution of 1:250 in blocking buffer for one hour at room temperature, washed with 1X TBS Tween 20 Buffer (Product # 28360), followed by incubation with DyLight 488 goat anti-mouse IgG secondary antibody (green,Product # A28175) at a dilution of 1:2000 and Hoechst 33342 dye (blue,Product # 62249) at a dilution of 1:5000 for 30 minutes at room temperature. Images were taken on a Thermo Scientific ToxInsight at 20X magnification. HP1 beta Antibody (MA3-053) in WBWestern blot was performed using Anti- HP1 Monoclonal Antibody(Product # MA3-053) and a 25 kDa band corresponding to HP1 was observed across the cell lines and tissues tested. Modified whole cell extracts (1% SDS) (30ug lysate) of HeLa (Lane 1), MCF7 (Lane 2), A-431 (Lane 3), U-2 OS (Lane 4), PC-3 (Lane 5), Raji (Lane 6), IMR32 (Lane 7), NIH/3T3 (Lane 8), Hep G2 (Lane 9), Mouse Liver (Lane 10) and Rat Liver (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

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